investigation of listeria monocytogenes and listeria ivanovii infection in wild cage birds in shahrekord by pcr
نویسندگان
چکیده
most birds probably can carry listeria spp. asymptomatically in their intestines with varying prevalence and can transmit the pathogens to human. the genus-listeria has two pathogenic species namely, l. monocytogenes and l. ivanovii. l. monocytogenes is a well-known cause of abortion, encephalitis and septicaemia in man and animals. l. ivanovii is an animal pathogen and in rare cases cause human infection. hence, this study was conducted to determine the occurrence of pathogenic l. monocytogenes and l. ivanovii in wild cage birds faeces from shahrekord. altogether 103 samples of bird faeces were collected with sterile cotton swabs from different areas of shahrekord, iran. swabs were placed directly into listeria enrichment broth. secondly enrichments were streaked on palcam agar. then evaluated to detect l. monocytogenes and l. ivanovii by polymerase chain reaction (pcr) method. neither l. monocytogenes nor l. ivanovii were found in birds of this study. the present result suggested that wild cage birds may have no role in the transmission of l. monocytogenes and l. ivanovii in the region.
منابع مشابه
evaluation of listeria monocytogenes and listeria ivanovii infection in cage birds in yazd, iran by polymerase chain reaction
background most birds probably can carry listeria spp. asymptomatically in their intestines with varying prevalence and can transmit the pathogens to humans. the genus-listeria has two pathogenic species namely, listeria monocytogenes and listeria ivanovii. of these, l. monocytogenes is a well-known cause of abortion, encephalitis and septicaemia in man and animals. listeria ivanovii is an anim...
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Listeria monocytogenes is a Gram-positive, facultative intracellular and invasive bacterium that has tropism to the placenta, and causes fetal morbidity and mortality in several mammalian species. While infection with L. monocytogenes and L. ivanovii are known as important causes of abortion and reproductive failure in cattle, the pathogenesis of maternal-fetal listeriosis in this species is po...
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The listeriolysin gene from a weakly hemolytic but virulent strain of Listeria monocytogenes serotype 1/2a was cloned in Escherichia coli K-12. Recombinants were identified on the basis of their cross-reactivities to hyperimmune antisera raised against streptolysin O and listeriolysin. Low levels of hemolytic activity were detected in crude lysates of strains harboring the listeriolysin gene. I...
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In culture supernatants of both Listeria ivanovii and Listeria monocytogenes Sv4b, for the first time a hemolysin of molecular weight 58 kDa was identified, which had all the characteristics of an SH-activated cytolysin, and which was therefore identified as listeriolysin O (LLO). In the case of L. ivanovii a second major supernatant protein of molecular weight 24 kDa co-purified with LLO. Howe...
متن کاملmolecular detection of listeria monocytogenes and l. ivanovii from bovine abortion in shahrekord
abortion can lead to major economic losses. l. monocytogenes and l. ivanovii can cause infertility and abortion. considering the importance of what mentioned, present study was conducted to detection of l. monocytogenes and l. ivanovii in bovine abortion from shahrekord, iran. vaginal discharge from 36 aborted cows were investigated to detection of l. monocytogenes and l. ivanovii. the sample...
متن کاملListeria monocytogenes serotype identification by PCR.
Serotyping is a universally accepted subtyping method for Listeria monocytogenes. Identification of the strain serotype permits differentiation between important food-borne strains (1/2a, 1/2b, and 4b) and provides a "gold standard" for comparing isolates analyzed in different labs and with different techniques. Although an efficient enzyme-linked immunosorbent assay serotyping protocol was des...
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عنوان ژورنال:
دامپزشکیجلد ۲۶، شماره ۱، صفحات ۲۲-۲۵
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